Quality Control
Good laboratory practice dictates that positive and negative control samples are included with each batch of samples to be processed. Although several international studies have been conducted, there are few published reports of the results of inter-laboratory quality control tests for culture of MAP (Sockett et al., 1992; Ridge et al., 2010; Heuvelink et al., 2017).
Programmes to evaluate the accuracy and consistency of culture test outcomes between laboratories are conducted in several countries. In the USA this is managed by the National Veterinary Services Laboratory, Ames, Iowa. In Australia, annual inter-laboratory proficiency tests for paratuberculosis are conducted under guidance from the OIE reference laboratory in Melbourne (Gwodz, 2006) and are managed by the Australian National Quality Assurance Program for veterinary laboratories; participation is required for laboratory accreditation under ISO17025 administered by the National Association of Testing Authorities. Faecal culture tests are evaluated less often than serological tests. The degree of difficulty in procuring samples to represent the full spectrum of species and levels of MAP burden, standardizing samples, confirming viability and shipping faecal samples in good condition to multiple laboratories is a disincentive to the regular conduct of such tests for MAP cultivation.18.18
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