summary and future perspectives

A wide variety of organ culture and animal models has been developed to study HIV-1 replication and the pathogenesis of AIDS. Apoptosis induced by HIV-1 among infected and bystander cells is an important component of the pathogenic process that leads to AIDS after HIV-1 infection.

Animal and organ culture models can also be used to test treatments for AIDS, including immune reconstitution and gene therapy. Thus, it will be interesting to see whether IL-7 treatment of HIV-1-infected FTOCs can protect ITTPs from HIV-1-mediated killing and enhance de novo naive T cell generation. Hematopoietic progenitor cells could be transduced with anti-HIV-1 or antiapoptotic genes and used to colonize FTOC or SCID-hu mice in order to check their effects on destructive events associated with HIV-1 infection of the thymus. Previous work has shown that transduction of human CD34⁺ cells with anti-HIV-1 genes inhibits HIV-1 replication in monocytes and lymphocytes produced in vitro.^204-209 Animal and organ culture models will continue to be vital for the study of HIV-1-mediated apoptosis and its prevention.

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