Recommendations and Concluding Thoughts

Summary recommendations based on cur­rently available test modalities are presented in Box 20.1. Priorities for future research include the characterization of immune responses in prospective studies spanning the lifetime of the animals, and factors that are involved in the variation in immune responses.

References

Clark, D.L., Koziczkowski, J.J., Radcliff, R.P., Carlson, R.A. and Ellingson, J.L.E. (2008) Detection of Mycobacterium avium subspecies paratuberculosis: comparing fecal culture versus serum enzyme- linked immunosorbent assay and direct fecal polymerase chain reaction. Journal of Dairy Science 91(7), 2620-2627. DOI: 10.3168∕jds.2007-0902.

Collins, M.T. (2002) Interpretation of a commercial bovine paratuberculosis enzyme-linked immunosorb­ent assay by using likelihood ratios. Clinical and Diagnostic Laboratory Immunology 9(6), 1367-1371. DOI: 10.1128∕cdli.9.6.1367-1371.2002.

Coussens, P.M. (2001) Mycobacterium paratuberculosis and the bovine immune system. Animal Health Research Reviews 2(2), 141-162. DOI: 10.1079∕AHRR200134.

Jungersen, G., Huda, A., Hansen, J.J. and Lind, P. (2002) Interpretation of the gamma interferon test for diagnosis of subclinical paratuberculosis in cattle. Clinical and Diagnostic Laboratory Immunology 9(2), 453-460. DOI: 10.1128∕cdli.9.2.453-460.2002.

Jungersen, G., Grell, S.N., Clemensen, A., Roust, T. and Howard, C.J. (2005) Interleukin-12 potentiation of the interferon-gamma test rescues day-old blood samples for the diagnosis of paratuberculo­sis. In: Manning, E.J.B. and Nielsen, S.S. (eds) Proceedings of the 8th International Colloquium on Paratuberculosis. International Association for Paratuberculosis, Madison, Wisconsin, pp. 501-506.

Kalis, C.H.J., Collins, M.T., Hesselink, J.W. and Barkema, H.W. (2003) Specificity of two tests for the early diagnosis of bovine paratuberculosis based on cell-mediated immunity: the Johnin skin test and the gamma interferon assay. Veterinary Microbiology 97(1-2), 73-86. DOI: 10.1016∕j.vetmic.2003.07.003.

Koets, A.P., Rutten, V.P., de Boer, M., Bakker, D., Valentin-Weigand, P. et al. (2001) Differential changes in heat shock protein-, lipoarabinomannan-, and purified protein derivative-specific immunoglobulin G1 and G2 isotype responses during bovine Mycobacterium avium subsp. paratuberculosis infec­tion. Infection and Immunity 69(3), 1492-1498. DOI: 10.1128/IAI.69.3.1492-1498.2001.

Kudahl, A.B., Nielsen, S.S. and Ostergaard, S. (2008) Economy, efficacy, and feasibility of a risk-based control program against paratuberculosis. Journal of Dairy Science 91(12), 4599-4609. DOI: 10.3168/ jds.2008-1257.

Li, L., Bannantine, J.P., Campo, J.J., Randall, A., Grohn, Y.T. et al. (2017) Identification of sero-reactive an­tigens for the early diagnosis of Johne's disease in cattle. PLoS ONE 12(9), e0184373. DOI: 10.1371/ journal.pone.0184373.

Magombedze, G., Shiri, T., Eda, S. and Stabel, J.R. (2017) Inferring biomarkers for Mycobacterium avi­um subsp. paratuberculosis infection and disease progression in cattle using experimental data. Scientific Reports 7, 44765. DOI: 10.1038∕srep44765.

Meyer, A., Bond, K., Van Winden, S., Green, M. and Guitian, J. (2018) A probabilistic approach to the interpretation of milk antibody results for diagnosis of Johne's disease in dairy cattle.

Mikkelsen, H., Jungersen, G. and Nielsen, S.S. (2009) Association between milk antibody and interferon-gamma responses in cattle from Mycobacterium avium subsp. paratuberculosis in­fected herds. Veterinary Immunology and Immunopathology 127(3-4), 235-241. DOI: 10.1016/j. vetimm.2008.10.315.

Muskens, J., van Zijderveld, F., Eger, A. and Bakker, D. (2002) Evaluation of the long-term immune re­sponse in cattle after vaccination against paratuberculosis in two Dutch dairy herds. Veterinary Microbiology 86(3), 269-278. DOI: 10.1016/S0378-1135(02)00006-8.

Nielsen, S.S. (2009) Use of diagnostics for risk-based control of paratuberculosis in dairy herds. In Practice 31(4), 150-154. DOI: 10.1136/inpract.31.4.150.

Nielsen, S.S. and Toft, N. (2006) Age-specific characteristics of ELISA and fecal culture for purpose­specific testing for paratuberculosis. Journal of Dairy Science 89(2), 569-579. DOI: 10.3168/jds. S0022-0302(06)72120-8.

Nielsen, S.S. and Toft, N. (2008) Ante mortem diagnosis of paratuberculosis: a review of accuracies of ELISA, interferon-gamma assay and faecal culture techniques. Veterinary Microbiology 129(3-4), 217-235. DOI: 10.1016/j.vetmic.2007.12.011.

Nielsen, S.S., Enevoldsen, C. and Grohn, Y.T. (2002a) The Mycobacterium avium subsp. paratuberculosis ELISA response by parity and stage of lactation. Preventive Veterinary Medicine 54(1), 1-10. DOI: 10.1016/S0167-5877(02)00008-9.

Nielsen, S.S., Gronbaek, C., Agger, J.F. and Houe, H. (2002b) Maximum-likelihood estimation of sen­sitivity and specificity of ELISAs and faecal culture for diagnosis of paratuberculosis. Preventive Veterinary Medicine 53(3), 191-204. DOI: 10.1016/S0167-5877(01)00280-X.

Nielsen, S.S., Krogh, M.A. and Enevoldsen, C. (2009) Time to the occurrence of a decline in milk produc­tion in cows with various paratuberculosis antibody profiles. Journal of Dairy Science 92(1), 149-155.

Nielsen, S.S., Toft, N. and Okura, H. (2013) Dynamics of specific anti-Mycobacterium avium subsp. para­tuberculosis antibody response through age. PLoS ONE 8(4), e63009. DOI: 10.1371/journal.pone. 0063009.

OIE (2003) Validation and certification of diagnostic assays. Resolution no. XXIX by the International Committee to the OIE. World Organisation for Animal Health (OIE), Paris, France.

Ransohoff, D.F. and Feinstein, A.R. (1978) Problems of spectrum and bias in evaluating the effi­cacy of diagnostic tests. New England Journal of Medicine 299(17), 926-930. DOI: 10.1056/ NEJM197810262991705.

Sergeant, E.S.G., Nielsen, S.S. and Toft, N. (2008) Evaluation of test-strategies for estimating probability of low prevalence of paratuberculosis in Danish dairy herds. Preventive Veterinary Medicine 85(1-2), 92-106. DOI: 10.1016/j.prevetmed.2008.01.005.

Sweeney, R.W., Whitlock, R.H., Buckley, C.L. and Spencer, P.A. (1995) Evaluation of a commercial enzyme-linked immunosorbent assay for the diagnosis of paratuberculosis in dairy cattle. Journal of Veterinary Diagnostic Investigation 7(4), 488-493. DOI: 10.1177/104063879500700411.

Toft, N., Nielsen, S.S. and Jorgensen, E. (2005) Continuous-data diagnostic tests for paratubercu­losis as a multistage disease. Journal of Dairy Science 88(11), 3923-3931. DOI: 10.3168/jds. S0022-0302(05)73078-2.

Wang, C., Turnbull, B.W., Grohn, Y.T. and Nielsen, S.S. (2006) Estimating receiver operating characteris­tic curves with covariates when there is no perfect reference test for diagnosis of Johne's disease. Journal of Dairy Science 89(8), 3038-3046. DOI: 10.3168∕jds.S0022-0302(06)72577-2.

Weber, M.F., Verhoeff, J., van Schaik, G. and van Maanen, C. (2009) Evaluation of Ziehl-Neelsen stained fae­cal smear and ELISA as tools for surveillance of clinical paratuberculosis in cattle in the Netherlands. Preventive Veterinary Medicine 92(3), 256-266. DOI: 10.1016∕j.prevetmed.2009.08.017.

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